Testosterone is synthesized from cholesterol. Cholesterol is a lipid — a fat. Diets severely restricted in dietary fat produce measurably lower testosterone levels in men, and the relationship is dose-dependent: as dietary fat intake decreases below approximately 15% of total calories, testosterone production declines progressively.
The landmark research on this relationship includes a study in the American Journal of Clinical Nutrition that found men who switched from a high-fat diet (40% of calories from fat) to a low-fat diet (25% of calories from fat) experienced a significant reduction in testosterone levels within six weeks.
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